Generation of a Ustilago maydis ade2 Mutant
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چکیده
منابع مشابه
Generation of a Ustilago maydis ade2 Mutant
The need exists to create the auxotrophic mutants for basidiomycete Ustilago maydis to allow selection of gene transformants in minimal growth media. The ADE2 gene was identified by homology with Saccharomyces cerevisiae. Adenine-requiring auxotrophic mutants were effectively created by homologous recombination in protoplasts using a standard plasmid containing the ADE2 locus interrupted by a p...
متن کاملسیتولوژی Ustilago maydis
رفتار هسته در تلیوسپور، بازیدیوسپور و میسلیومهای Ustilago maydis مورد بررسی قرار گرفت. جهت کشت تلیوسپورها از محیط PDA + 10% دکستروز استفاده گردید. مطالعه رفتار هسته با استفاده از میکروسکوپ اپیفلورسنت و رنگآمیزی با دو رنگ اتیدیوم برماید و اکردین اورنج صورت پذیرفت. هسته در تلیوسپور کروی و بزرگ و اکثر فضای داخلی آن را اشغال نموده است. بازیدیوسپورها (اسپوریدیوم) به صورت غیرانگلی و به روش جوانهز...
متن کاملUstilago maydis as a Pathogen.
The Ustilago maydis-maize pathosystem has emerged as the current model for plant pathogenic basidiomycetes and as one of the few models for a true biotrophic interaction that persists throughout fungal development inside the host plant. This is based on the highly advanced genetic system for both the pathogen and its host, the ability to propagate U. maydis in axenic culture, and its unique cap...
متن کاملGlycolysis in Ustilago maydis.
The kinetic parameters of the 10 glycolytic enzymes and glycolytic fluxes were determined for the first time in Ustilago maydis. Enzyme activities in yeast grown in minimal medium and harvested in the stationary stage were twofold higher than those from yeast grown in rich medium. In contrast, in yeast harvested in the exponential stage, the enzyme activities were higher in cells grown in rich ...
متن کاملcytology of ustilago maydis
cytology of ustialgo maydis teliospore, basidiospore and mycelium was investigated with epiflouresent microscope using both ethidium bromide and ocridin orange. surface sterilized teliospores were cultured on pda + 10% dextrose medium and incubated at 25ºc for 5 days. the results revealed that nucleus in teliospore occupied most of the inner part of its cell. sporidia had an ellipsoid shape nuc...
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ژورنال
عنوان ژورنال: Fungal Genomics & Biology
سال: 2016
ISSN: 2165-8056
DOI: 10.4172/2165-8056.1000138